bose forward and reverse primers to amplity gene x 5'ATTAGTCATGCCAACTTGCACTGATATGA...geneX...CCAGGCGCTAACCTAGATCGCTAGTATCG3' Forward primer: A. 5'-AGTCACGTTCAACCGTACTG-3' B. 5'-GTCATGCCAACTTGCACTGA-3' C. 5'-TCAGTGCAAGTTGGCATGAC-3' D. 5'-CAGTACGGTTGAACGTGACT-3' E. other? Reverse primer: A. 5'-TGATCGCTAGATCCA CGC-3 B. 5'-CGCTAACCTAGATCGCTAGT-3' C. 5'-ACTAGCGATCTAGGTTAGCG-3' D. 5'-GCGATTGGATCTAGCGATCA-3' E. other? * Sequences above are 20mers with 50%GC.

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Choose forward and reverse primers to amplify gene X
5'ATTAGTCATGCCAACTTGCACTGATATGA...geneX...CCAGGCGCTAACCTAGATCGCTAGTATCG3'
Forward primer:
A.
5'-AGTCACGTTCAACCGTACTG-3'
B. 5'-GTCATGCCAACTTGCACTGA-3'
C. 5'-TCAGTGCAAGTTGGCATGAC-3'
D. 5'-CAGTACGGTTGAACGTGACT-3'
E. other?
Reverse primer:
A. 5'-TGATCGCTAGATCCAATCGC-3'
B. 5'-CGCTAACCTAGATCGCTAGT-3'
C. 5'-ACTAGCGATCTAGGTTAGCG-3'
D. 5'-GCGATTGGATCTAGCGATCA-3'
E. other?
* Sequences above are 20mers with 50%GC.
Transcribed Image Text:Question Choose forward and reverse primers to amplify gene X 5'ATTAGTCATGCCAACTTGCACTGATATGA...geneX...CCAGGCGCTAACCTAGATCGCTAGTATCG3' Forward primer: A. 5'-AGTCACGTTCAACCGTACTG-3' B. 5'-GTCATGCCAACTTGCACTGA-3' C. 5'-TCAGTGCAAGTTGGCATGAC-3' D. 5'-CAGTACGGTTGAACGTGACT-3' E. other? Reverse primer: A. 5'-TGATCGCTAGATCCAATCGC-3' B. 5'-CGCTAACCTAGATCGCTAGT-3' C. 5'-ACTAGCGATCTAGGTTAGCG-3' D. 5'-GCGATTGGATCTAGCGATCA-3' E. other? * Sequences above are 20mers with 50%GC.
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