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This is an SDS-PAGE of an antibody purification sample with IgG seperated from a Bovine Calf serum. Would you be able to describe the bands that are appearing and why it appeared on the gel this way? As well, a western blot was done after the SDS-PAGE and the bands that appeared were at 50 kDa and 150 kDa for the IgG and two elution lanes. May you please explain?
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- 250- 1 2 3 4 5 6 -250 150- -150 100- -100 75- -75 50- 37- 25- 20- 15- 10 10- Std Biorad BSA Calf serum Fibrinogen Std Biorad Transferrin 161-0318 161-0374 -50 -37 -25 -20 15 10 2. Measure the distance (in mm) moved for each protein standard band and record in Table3.6 below. Table 3.6 Example of table for recording of data - measured distance moved and calculated Rr values Item Band Distance moved (mm) Rf Log 10 Mr (from graph) Calculated Mr (antilog of the previous column) Sample C Band 1 Sample F Band 1 Band 2 Sample B Band 1 Band 2 Sample T Band 1Based on the image below, select the correct statement. Complex II QH₂ Q- 10 2 HO 2 HO Fe-S (2.8 FADH₂ FAD- Succinate Fumarate https://canvas.uts.edu.au/assessment questions/356986/files/1562694/download? 2e verifier-eUTT3hYal2YYTWlywV8TIFA3USmzCsM52jECmvTo O Succinate is reduced to fumarate O Succinate is oxidised to FAD O The Fe-S center shuffles electrons from FAD to ubiquinone (Q) O The Fe-S center shuffles electrons from FADH2 to ubiquinone (Q) The Fe-S center shuffles electrons from FADH2 to ubiquinonol (QH2) W 88 16°C11 Ⓡ The tissue type that is shown in this image is Saved LM 1000x Be as specific as possible. +
- EcoRI 4359 Aatll - Zral 4284 Bei 4209 BsrBl 4205 Clal - BspDI 23 Hindill 29 EcoRV 185 Bmtl - Nhel 229 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Hincll 3905 Scal 3844 BamH 375 Sgrl 409 Banll 471 Banll 485 Вы 3787 Bsl 3759 Bgl 528 Sphl 562 EcoNI 622 Sal - Acct - Hincll 651 Pvul 3733 Pstl 3607 Bartl 3602 Pshl 712 Asel 3537 Eagl 909 Bell 949 Bsal 3433 BarDI 3420 Nrul 972 PBR322 4,361 bp BstAPI 1045 Ahdi 3361 BspMI - Bfual 1063 PAMI 1315 Bsml 1353 PAMI 1364 Acul 3000 Ori Styl 1369 Aval - BsoBl 1425 PpuMI 1438 Msel 1444 Bigl 1447 Ppu 1480 AlwNI 2884 Bell 2777 kI 2682 rop Drdi 2575 Bsgl 1650 BspEI 1664 Pcil - Afl 2473 rBl 2404 Earl 2351 Bspol - Sapl 2350 Ndel 2295 BstAPI 2291 Bsaß 1668 Xmat 2029 Pvull 2064 BsmBI 2122 Dndl 2162 BstZ171 - Acct 2244 Bsal 2225 TthI- PI 2217 Figure 1 If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.in Course: 23SPCMP Anat & Phys... The tissue type that is indicated by the black line is @ # M Question 32 - Lab Practical 1 -... $ % LM 320x 4+Ad 1 of 2 · 0:01 lazada.com.ph/ Ad will end in 2 ---- 1. Make a summary table of the 3 sections of theprimitive gut tube, indicating the blood supply andthe adult derivatives of each section 2. Describe the following congenital anomaliesinvolving the digestive system: a. Esophageal atresiab. Malrotations of the midgutc. Imperforate anus 3. Briefly discuss the pharyngeal pouches and their derivatives. 4. Summarize the 5 stages of fetal lungdevelopment 5. Briefly discuss the role of pulmonary surfactant inneonatal adaptation.
- orY i 8 < Bilirubin is converted from unconjugated into conjugated before entering the liver. False O True O dilayl haaWhat is the difference between Graves disease and Cushing disease? +66-t=449595&cmid=1668389 textb... My ATI NOLOGY SUPPORT ✓ MTH 208-01 (MW... K Kaplan Login WP WileyPLUS VitalSource Books... Zika virus is chiefly spread using an insect vector, more specifically, mosquitoes. Given this, which of the following helps account for why all blood donations in J.S. are screened for Zika? O a. Zika can be easily transmitted from person to person even through incidental contact O b. Zika infections are now common and regularly reported in every U.S. State (except Alaska) O c. Screening of blood donations is the main way in which Zika infections are diagnosed in the U.S. O d. Climate change extends the habitat of mosquitoes that can carry the virus Next page
- Chest x-rays give the lowest doses. Why?A- B ↑ ↑ C- An image of a hematocrit blood test is shown. Indicate which layer refers to the buffy coat. A B Ос1 10 20 30 40 50 60 70 -I---- 5' АTCGGTCТCGGCTACTACАТАAАСGCGCGCATATATCGAТАТСТАGСТАGСТАТCGGTCTAGGCTACTАC 3' TAGCCAGAGCCGATGATGTATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGATCCGATGATG I--------I--- --I--- --I------ --I--- -I---------I Promoter 80 90 100 110 120 130 140 -I---- 5' CAGGTATсGGTCTGATCTAССТAGCTTCTтсттстстстстсссссGCGGGGGCTGTACTATСATGCGTCG 3' GTCCATAGCCAGACTAGATCGATCGAAGAGAAGAGAGAGAGGGGGCGCCCCCGACATGATAGTACGCAGC -I- -I------- -I--- -I---------I RBS 150 160 170 180 190 200 210 -----I--- ---I-- ------I- ---I---------I---- -I---------I 5' тстCGGCTАСТАCGTAAACGCGCGCATATAтCGATATCTAGCТAGСТАТСGGTстCGGCTACTAсGTAAA 3' AGAGCCGATGATGCATTTGCGCGCGTATATAGCTATAGATCGATCGATAGCCAGAGCCGATGATGCATTT 220 230 240 250 --I----- ---I-- ------I--- -I 5' CCCTATTAGCATGGGTCATATTTGTGTCTGCTTGTTGGGT 3' GGGATAATCGTACCCAGTAGAAACACAGACGAAGAACCCA a. What are the nucleotides of the MRNA from gene Z? b. What are the amino acids encoded by gene Z? ( Vate V
