[1-14C]Ribose 5-phosphate is incubated with a mixture of purified transketolase, transaldolase,phosphopentose isomerase, phosphopentose epimerase, and glyceraldehyde 3-phosphate. Predictthe distribution of radioactivity in the erythrose 4-phosphate and fructose 6-phosphate that wereformed in this system.Diagram the reactions involved, showing the structures of each intermediate compound in thesystem and clearly indicating the location of the 14C in each molecule.

The pentose phosphate pathway consists of 2 phases; Oxidative and Non-oxidative phase. In the…

Answered: [1-14C] Ribose 5-phosphate is incubated…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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[1-14C]Ribose-5-phosphate is incubated with a mixture of purified transketolase, transaldolase, phosphopentose isomerase, phosphopentose epimerase, and glyceraldehyde-3-phosphate. Predict the distribution of radioactivity in the erythrose-4-phosphate and fructose-6-phosphate that are formed in this mixture.
Draw the products of the reaction of xylulose-5-phosphate and erythrose-4-phosphate catalyzed by transketolase in the pentose phosphate pathway. Provide the structure in the protonation state found in physiological conditions. H H H OH FO HO-H H-OH H OPO3²- Q transketolase Draw glyceraldehyde-3- phosphate H H- H H H O OH OH OPO3²- Draw fructose-6- phosphate Q I I
The mechanism involved in the reaction catalyzed by phosphoglyceromutase is known to involve a phosphorylatedenzyme intermediate. If 3-phosphoglycerate is radioactively labeledwith 32P, the product of the reaction, 2-phosphoglycerate, does nothave any radioactive label. Design a mechanism to explain these facts.
Certain microorganisms with a modified citric acid cycle decarboxylate a-ketoglutarate to produce succinate semialdehyde: (a) Succinate semialdehyde is then converted to succinate, which is furthermetabolized by standard citric acid cycle enzymes. What kind of reactionis required to convert succinate semialdehyde to succinate? Showany coenzymes that might be involved.(b) Based on your answer in part a, how does this pathway compare to thestandard citric acid cycle in energy yield?
Refer to Figure, which indicates ∆G for each glycolytic reaction under intracellular conditions. Assume that glyceraldehyde-3-phosphate dehydrogenase was inhibited with iodoacetate, which reacts with its active site cysteine sulfhydryl group. Which glycolytic intermediate would you expect to accumulate most rapidly, and why?
Normal carbonic anhydrase has zinc at its active site . Suggest a structural reason why carbonicanhydrase synthesized with Ni²⁺, Fe²⁺, or Mn²⁺ in place of Zn²⁺ gives an enzyme with less catalytic efficiency.
The krebs cycle has 8 enzymes: citrate synthase, aconitase, isocitrate dehyrogenase, a-ketoglutarate dehydrogenase, succinyl –CoA synthease, succinate dehyrogenase, fumarase and malate dehyrogenase. a)Write name the cofactors required by each enzyme reaction. b)For each enzyme determine which of the following deascribes the type of reactions catalyzed: condensation (Carbon-carbon bond formation), dehydration (Loss of water), hydration (addition of water), decarboxylation (loss of CO2), oxidation-reduction; subsrate level phospohorylation; isomerization.
Consider a series of experiments in which 32P-labeled inorganic phosphate were introduced to erythrocytes (red blood cells) undergoing glycolysis. Would you expect to see the labeled phosphate in any glycolytic intermediates? If so, which and at what relative concentrations?
Hexokinase catalyzes the first step of glycolysis, in which glucose is phosphorylated to form glucose‑6‑phosphate. Give two reasons why a Mg2+ cation is required to facilitate this reaction.
Certain microorganisms with an incomplete citric acid cycle decarboxylate α-ketoglutarate to produce succinate semialdehyde. A dehydrogenase then converts succinate semialdehyde to succinate. These reactions can be combined with other standard citric acid cycle reactions to create a pathway from citrate to oxaloacetate. Compare the ATP and reduced cofactor yield of the standard and alternate pathways.
Write out the balanced chemical equation for the FIRST round of oxidation of C16:cis-9.
Using the principles described in the text regarding pyridoxal phosphate mechanisms, propose a mechanism for the reaction catalyzed by serine hydroxymethyltransferase.

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