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- Remember that although there are many interesting ideas about genetic engineering of plants and animals, this is specifically about GE bacteria. Please be sure you are answering the following questions 3) What are the benefits (or potential benefits) of the engineered bacterium? 4) What are the risks (or potential risks) of the engineered bacterium?1. Compare Illumina/Pacbio/Nanopore sequencing methods.2. How do you construct the profile matrix? Answer all the part in details and with proper examples.1. Explanation of the benefits of bioinformatics in biological sciences. 2. explain the relationship between the position of the role of bioinformatics in the field of biology.3. explain the main principles of bioinformatics as a tool in the field of biotechnology.4. Mention examples of bioinformatics that are now a current issue.5. Look for case studies based on online/printed newspaper articles related to the role of bioinformatics in Indonesia and provide feedback.
- 1.Explain the purpose and overall technique for the following biotechnology applications: a. Isolation of Nucleic Acids b. Gel electrophoresis c. Polymerase Chain Reaction (PCR)Briefly discuss the following topics, including appropriate examples for each:3.1. Genomic fingerprinting for the phylogenetic analysis of bacteria 3.2. Photosynthetic pigments and environmental habitats of green sulphur bacteria3.3. Advantages of phage therapy for bacterial infections1.Discuss how PCR may be used for the detection of disease-causing pathogens in a population during the COVID Pandemic. For example: it may be used to check if a patient has a COVID virus infection. 2. Discuss how the cloning and expression of certain genes allows for massive production of the desired product. For Example: the cloning and expression of insulin in bacteria allows for the mass production of this necessary protein for use by diabetic patients.
- 1. Explain why the 16S rRNA gene sequencing is suitable for bacterial identification in general and why it is mostly limited to genus level.present briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.A. What are the 4 “Koch’s Postulates”? B. Are they still valid today? How would we use them to establish the relationship between a specific microbe and a disease? Can we substitute such things as finding antibodies or DNA traces for some steps? If so, why? C. How has microbiology contributed to the advancement in molecular biology?
- A. DNA Sequencing 1. Determine the base sequences of the sample DNA from unknown organisms by examining the bands after gel electrophoresis on photographic films. a. Unknown 1 b. Unknown 2 AGT C I I c. Unknown 3 ||| || | | AGT | || | ||| | | | || I || || ull с I AGT 2. Write down the DNA sequences of each Unknown. I I || d. Unknown 4 || |||||| AGT || ||| | || I || ||| с C ||1. List down 20 products produced using Traditional Biotechnology and 10 products made using Modern Biotechnology. Be sure to include the organism responsible in the production of each product.explain how the following genotypic andphenotypic methods of microbe identification is done. Use diagrams or schematic representations to explain yourwork. 1. Pulsed-Field Gel Electrophoresis (PFGE)2. Whole Genome Sequencing (WGS)