See the Light week 2

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School

University of Nebraska, Lincoln *

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Course

110L

Subject

Chemistry

Date

May 13, 2024

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pdf

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5

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Experiment 5 See the Light – Week 2 Total Points: 78 Student’s Name: Parker Gould Lab Section: 613 Report Your report should include the following: Data tables 5-4 and example calculations, 5-5, 5-6, 5-8 and example calculations, 5-9 and example calculations Absorption spectrum for each mixture (Red-Yellow, Red-Blue, Yellow-Blue, and all three dyes) (5pts/graphs) Absorption spectrum for unknown (5 pts) Answers to questions NOTE: You must show your work for all calculations; no work, no credit. DATA TABLE 5-4. Concentration of Mixtures of Known Concentration (4 pts) Mixture Red #40 (M) Yellow #5 (M) Blue #2 (M) Red-Yellow 1.51x10^- 4 1.405x10^ -4 0 Red-Blue 1.51x10^- 4 0 1.61x10^-4 Yellow-Blue 0 1.405x10^ -4 1.073x10^- 4 All three dyes 1.007x10 ^-4 9.367x10^ -5 1.073x10^- 4 Measured in SpectroVis (known mixtures only) Example calculations must include all concentration calculations for the Red dye (Red-Yellow, Red-Blue, and all three dyes) (6 pts): M1V1=M2V2 Red-Yellow (3.02x10^-4)(10mL)=(M2)(20mL) 0.00302=M2(20mL) 0.00302/20 M2=1.51x10^-4 Red-Blue (3.02x10^-4)(10mL)=(M2)(20mL) 0.00302/20 M2= 1.51x10^-4 All 3 dyes (3.02x10^-4)(10mL)=(M2)(30mL) 0.00302=(M2)(30mL)
Experiment 5 See the Light – Week 2 Total Points: 78 0.00302/30 M2= 1.007x10^-4 DATA TABLE 5-5. Absorbances for Diluted Stock Solutions and Mixtures (5 pts) Dye Analytical Wavelength (nm) Red Yellow Blue Red-Ye llow Red-B lue Yellow- Blue All Three Dyes Red #40 539.30 0.375 0.078 0.267 0.237 0.305 0.175 0.190 Yellow #5 426.10 0.255 0.509 0.136 0.369 0.199 0.344 0.295 Blue #2 610.30 -0.03 0 0.078 0.551 0.023 0.167 0.337 0.119 DATA TABLE 5-6. Unknown (3 pts) Dye Present in Unknown (circle) Analytical Wavelength (nm) Absorption Red #40 Yes No 539.30 0.305 Yellow #5 Yes No 426.10 0.075 Blue #2 Yes No 610.30 0.123 DATA TABLE 5-8. Concentrations in Unknown (6 pts) Dye Concentration (M) Red #40 2.318x10^-4 Yellow #5 5.343x10^-5 Blue #2 1.000x10^-4 (3 pts) Calculated using Beer’s Law ε from Table 5-7 A from Table 5-6
Experiment 5 See the Light – Week 2 Total Points: 78 Example calculation must include concentration calculations for all dyes in the unknown. (3 pts) A=Ebc Red 0.305=(1315.7)(1)(c) 0.305/1315.7= 2.318x10^-4 Yellow 0.075=(1403.7)(1)(c) 0.075/1403.7 5.343x10^-5 Blue 0.123=(1229.5)(1)(c) 0.123/1229.5 1.000x10^-4 DATA TABLE 5-9. Concentration in Mixtures from Absorbance and Percent Error from Known (10 pts) Mixture Dye Known Concentration (M) (Calculated by dilutions of diluted stock solutions) Calculated Concentration (M) (Calculated from Beer’s Law) Percent Error Red-Yellow Red 1.51x10^-4 1.801x10^-4 19.27% Yellow 1.405x10^-4 2.629x10^-4 87.12% Red-Blue Red 1.51x10^-4 2.318x10^-4 53.51% Blue 1.61x10^-4 1.358x10^-4 -15.65% Yellow-Blue Yellow 1.405x10^-4 2.451x10^-4 74.45% Blue 1.61x10^-4 2.741x10^-4 70.25% All Three Red 1.007x10^-4 1.444x10^-4 43.40% Yellow 9.367x10^-5 2.012x10^-4 114.83% Blue 1.073x10^-4 9.679x10^-5 -9.790%
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